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Optical tweezers for the micromanipulation of plant cytoplasm and organelles
Authors:Hawes Chris  Osterrieder Anne  Sparkes Imogen A  Ketelaar Tijs
Institution:1 School of Life Sciences, Oxford Brookes University, Oxford, OX3 0BP, UK;2 Laboratory of Plant Cell Biology, Wageningen University, Droevendaalsesteeg 1, Wageningen, 6708PB, The Netherlands
Abstract:Laser trapping of micron-sized particles can be achieved utilizing the radiation pressure generated by a focused infrared laser beam. Thus, it is theoretically possible to trap and manipulate organelles within the cytoplasm and remodel the architecture of the cytoplasm and membrane systems. Here we describe recent progress, using this under utilized technology, in the manipulation of cytoplasmic strands and organelles in plant cells.
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