Identification and characterization of glucagon-like peptide-1 7-36 amide-binding sites in the rat brain and lung |
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| Authors: | S M Kanse B Kreymann M A Ghatei S R Bloom |
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| Institution: | Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London, England. |
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| Abstract: | High-affinity binding sites for glucagon-like peptide-1 7-36 amide (GLP-1 7-36 NH2) were identified in rat brain and lung membranes. Binding of 125I]GLP-1 7-36 NH2 was rapid, reversible, specific, saturable and pH dependent. Specific binding in the central nervous system was particularly high in the hypothalamus and the brain stem. Oxyntomodulin, glucagon-like peptide-1, glucagon-like peptide-2 and glucagon were 100-1000-fold less potent than GLP-1 7-36 NH2 in competition for this binding site. |
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