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SNP-Ratio Mapping (SRM): Identifying Lethal Alleles and Mutations in Complex Genetic Backgrounds by Next-Generation Sequencing |
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| Authors: | Lindner Heike Raissig Michael T Sailer Christian Shimosato-Asano Hiroko Bruggmann Rémy Grossniklaus Ueli |
| Affiliation: | Institute of Plant Biology and Zürich-Basel Plant Science Center, University of Zürich, CH-8008 Zürich, Switzerland. |
| Abstract: | We present a generally applicable method allowing rapid identification of causal alleles in mutagenized genomes by next-generation sequencing. Currently used approaches rely on recovering homozygotes or extensive backcrossing. In contrast, SNP-ratio mapping allows rapid cloning of lethal and/or poorly transmitted mutations and second-site modifiers, which are often in complex genetic/transgenic backgrounds. |
| Keywords: | Arabidopsis thaliana second-site modifier mutation forward genetic screen genetic model systems lethal mutations |
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