| Abstract: | The ultrastructural and immunocytochemical study of rat male germ cells on different developing stages has been made. The investigation of morphological changes of spermatogenic cells has demonstrated the presence of tight connections between chromatoid bodies (CBs) and other cell organelles, particularly with the nucleus and Golgi apparatus; has revealed the association of manchette noncentrosomal microtubules (MT) with spermatid perinuclear ring plasma membrane (PM) in the zone of the adhesion intercellular contact--zonula adhaerens (ZA). The comparison of the results obtained in this work with available literary data has given possibility to analyze expected pathways of noncentrosomal MT nucleation in the late spermatids. This paper puts the supposition that noncentrosomal MTs are nucleated on the sites of perinuclear ring ZA. The immunocytochemical analysis discovered two novel proteins for these cells--BASP1 and MARCKS. It has been shown that these proteins present in the CBs in the early spermatids. During the spermatozoid differentiation these proteins are revealed along the outer dense fibers (ODFs) of the sperm tail. BASP1 and MARCKS are supposed to involve in the processes of calcium accumulation in the CBs and ODFs. Calcium ions seem to play the significant role in RNA processing and protein synthesis in spermatids. Calcium is also necessary for the mobility of sperms which is mainly determined by ODFs. |
