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A new selective pre-column ninhydrin-based derivatization for a RP-HPLC determination of plasma asymmetric dimethyl-l-arginine (ADMA) by fluorescence detection |
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| Authors: | Salvatore Sotgia Angelo Zinellu Gerard Aime Pinna Luca Deiana Ciriaco Carru |
| Affiliation: | (1) Department of Biomedical Sciences, Chair of Clinical Biochemistry, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy;(2) Department of Medicinal and Toxicological Chemistry, University of Sassari, Sassari, Italy;(3) National Laboratory of the National Institute of Biostructures and Biosystems, Osilo, Italy |
| Abstract: | We report a new selective and direct pre-column ninhydrin-based derivatization reaction for determination of plasma ADMA levels. This original derivatization procedure matched to a validated and rapid RP-HPLC method can be a useful alternative to other assays in which time consuming and expensive extraction and/or purification steps are required. |
| Keywords: | ADMA Homoarginine Ninhydrin Fluorescence |
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