A general method for the direct isolation of the 3'-terminal fragments derived from transfer RNA molecules |
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Authors: | R C Warrington |
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Affiliation: | Microbiology Section, Life Sciences, University of Connecticut, Storrs, Connecticut 06268 USA |
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Abstract: | A general procedure for the isolation of 3′-linked fragments derived from tRNA molecules is described. Purified N-2-naphthoxyacetylglycyl derivatives of the and of yeast were exhaustively digested with RNase T1 and the 3′-linked fragments (bearing the derivative) were separated from other degradation products (lacking the derivative) by stepwise chromatography on BD-cellulose. Subsequent chromatographic resolution and base-composition analysis allowed tentative identification of the 3′-terminals of and as Gp(Cp,Ap)CpCpA and Gp(Cp,Cp,Up,Ap)CpCpA, respectively. The potential utility of this procedure for development of a novel approach to nucleic acid sequence analysis is discussed. |
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