Isolation of a hybrid plasmid with homologous sequences to a transposing element of Drosophila melanogaster

In Drosophila several transposing elements that contain the white locus are known. Transpositions of one such element, which carries both the white-apricot (w^a) and the neighboring roughest (rst⁺) genes, have been isolated at more than 120 sites scattered over the entire genome (Ising and Ramel 1976). We have isolated a recombinant plasmid (61F4) containing sequences that appear to be present on this transposing element (TE). In nontransposed stocks, 61F4 hybridizes to approximately 40 sites in the polytene chromosomes including the nucleolus, the chromocenter and chromosome section 3C (that is, the white-apricot roughest region). In six different tranpositions tested, the genetic map position of the TE corresponds to one site of in situ hybridization of 61F4, indicating that the TE contains homologous sequences. The sites of in situ hybridization correlate with the w^a allele or alleles derived from w^a but not with w⁺ and other w alleles tested, nor with an X-ray-induced revertant of w^a. Thus w^a strains appear to carry additional DNA sequences homologous to 61F4, close to or within the w gene. The recombinant plasmid 61F4 carries 7.3 kb of Drosophila DNA inserted into pSF2124. It contains a segment homologous to a member of the copia gene family (Finnegan et al. 1978). Since copia appears to be a highly mobile element (Strobel, Dunsmuir and Rubin 1979), the association of copia sequences with the w^a-rst⁺ transposing element suggests that copia sequences may be responsible for the transposition of this element.