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Human cytomegalovirus DNA: BamHI, EcoRI and PstI restriction endonuclease cleavage maps |
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| Authors: | P J Greenaway J D Oram R G Downing K Patel |
| Institution: | Département de Biochimie, Faculté des Sciences et de Génie, Université Laval, Québec, Qué, G1K 7P4 Canada |
| Abstract: | The cloned HindIII fragments of human cytomegalovirus (HCMV) strain AD169 DNA were mapped with respect to the BamHI, EcoRI and PstI restriction endonuclease cleavage sites. Composite restriction endonuclease cleavage maps for the entire virus genome were constructed using the previously established linkages between the HindIII fragments. |
| Keywords: | Recombinant DNA cosmid pHC79 in vitro packaging transduction Ap ampicillin Gm gentamicin kb kilobase pairs Km kanamycin r-m restriction-modification Su sulfonamide Tc tetracycline Tm tobramycin |
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