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Characterization and application of a new aqueous two phase system based on gum acacia (ex.Acacia senegal), a naturally occuring polysaccharide,and polyethylene glycol
Authors:Alka Rastogi  Subhash Chand
Affiliation:(1) Biochemical Engineering Research Centre, Indian Institute of Technology, 110 016 New Delhi, India
Abstract:Summary Gum acacia has been used with polyethylene glycol to form a two phase aqueous polymeric system. The system followed Newtonian fluid behaviour and the acacia rich phase supported a high concentration of protein (>1.3 mg/ml) in solution. The data on partitioning of various proteins in this system indicates that it can be used for selective separation of a particular protein from a protein mixture.
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