Hyperthermophilic redox chemistry: a re-evaluation |
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| Authors: | Peter L Hagedoorn Martijn C.P.F Driessen Marieke van den Bosch Ilse Landa Wilfred R Hagen |
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| Affiliation: | Wageningen University and Research Centre, Department of Biomolecular Sciences, Bioinorganic Chemistry Group, Dreijenlaan 3, NL-6703 HA Wageningen, The Netherlands |
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| Abstract: | The redox chemistry of Pyrococcus furiosus rubredoxin and ferredoxin has been studied as a function of temperature in direct voltammetry and in EPR monitored bulk titrations. The Ems of both proteins, measured with direct voltammetry, have a normal (linear) temperature dependence and show no pH dependence. EPR monitoring is not a reliable method to determine the temperature dependence of the Em: upon rapid freezing the proteins take their conformation corresponding to the freezing point of the solution. |
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| Keywords: | Rubredoxin Ferredoxin Reduction potential Hyperthermophile Pyrococcus furiosus |
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