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A simple method for efficient separation and purification of c-phycocyanin and allophycocyanin from Spirulina platensis
Authors:Yi-Ming Zhang  Feng Chen
Affiliation:(1) Light Industry Department, Guizhou University of Technology, Guiyang, China;(2) Department of Botany, the University of Hong Kong, Pokfulam Road, Hong Kong
Abstract:c-Phycocyanin and allophycocyanin were separated and purified from Spirulina platensis by precipitation with ammonium sulphate, ion exchange chromatography and gel filtration chromatography. Pure c-phycocyanin and allophycocyanin were finally obtained with an A620/A280value of 5.06 and an A655/A280 value of 5.34, respectively.
Keywords:allophycocyanin  c-phycocyanin  purification  separation  Spirulina platensis
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