| 大豆疫霉菌的分离与鉴定 |
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| 引用本文： | 宋志刚,郭成亮,赵曙国,吴国贵,李英群,胡文多,葛婕,姚智慧. 大豆疫霉菌的分离与鉴定[J]. 菌物研究, 2008, 6(4): 208-210 |
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| 作者姓名： | 宋志刚 郭成亮 赵曙国 吴国贵 李英群 胡文多 葛婕 姚智慧 |
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| 作者单位： | 秦皇岛出入境检验检疫局,秦皇岛,066002;河北农业大学植物保护学院,保定,071000 |
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| 基金项目： | 国家质检总局课题 |
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| 摘 要： | 采用叶碟诱捕法从2007年进口的美国大豆携带的土壤和2006年从黑龙江感病大豆田采集的土壤中分离出2株疫霉菌菌株,并对病原菌进行了形态特征、致病性、分子检测。结果表明:形态观察为疫霉属真菌;接种大豆后出现典型的大豆疫病症状;采用大豆疫霉的特异性引物PCR检测,2个菌株均能扩增出分子量为330 bp的特异性条带。结合形态、致病性测定和分子检测,2株病菌鉴定为大豆疫霉菌(Phytophthora sojaeKauf-mann et Gerdemann)。
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| 关 键 词： | 大豆疫霉 分离 鉴定 分子检测 |
Isolation and Identification of Phytophthora sojae |
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| SONG Zhi-gang,GUO Cheng-liang,ZHAO Shu-guo,WU Guo-gui,LI Ying-qun,HU Wen-duo,GE Jie,YAO Zhi-hui. Isolation and Identification of Phytophthora sojae[J]. Journal of Fungal Research, 2008, 6(4): 208-210 |
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| Authors: | SONG Zhi-gang GUO Cheng-liang ZHAO Shu-guo WU Guo-gui LI Ying-qun HU Wen-duo GE Jie YAO Zhi-hui |
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| Affiliation: | SONG Zhi-gang1,GUO Cheng-liang1,ZHAO Shu-guo1,WU Guo-gui1,LI Ying-qun1,HU Wen-duo1,GE Jie1,YAO Zhi-hui2(1.Qinhuangdao Entry-Exit Inspection , Quarantine Bureau,Qinhuangdao 066002,China,2.College of Plant Protection,Hebei Agricultural University,Baoding 071000,China) |
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| Abstract: | Using the leaf-disk baits method,2 pathogenic isolates were obtained from the soil samples from the imported USA soybeans in 2007 and from Phytophthora sojae epidemic field of Heilongjiang province in 2006.Based on the morphological character,pathogenicity and specific PCR test,they are identified as Phytophthora sojae Kaufmann et Gerdemann.This method can be used as model for Phytophthora sojae identification from imported soybean. |
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| Keywords: | Phytophthora sojae isolation identification PCR test |
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