Studies on the mode of action of calciferol. XVIII. Evidence for a specific high affinity binding protein for 1,25 dihydroxyvitamin D3 in chick kidney and pancreas. |
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Authors: | S Christakos A W Norman |
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Institution: | Department of Biochemistry University of California Riverside, California 92521 USA |
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Abstract: | Cytosol fractions prepared from rachitic chick kidney and pancreas were analyzed for binding of vitamin D3 metabolites by sucrose density gradient centrifugation. Both cytosol fractions were found to contain a 3.6S macromolecule which specifically binds 1,25-dihydroxy3H] vitamin D3 and in addition a 5 to 6S macromolecule which binds 25-hydroxy3H]vitamin D3. Sucrose gradient analysis of a KCl extract prepared from kidney or pancreas chromatin resulted in a peak (3.6S) of bound 1,25-dihydroxyvitamin D3 which could not be distinguished from the cytoplasmic binding component. The interaction of 1,25-dihydroxy3H]vitamin D3 with the cytoplasmic binding component of both tissues occurred at low concentrations of hormone with high affinity. |
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Keywords: | To whom all reprint requests should be addressed |
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