Plant regeneration from somatic embryogenic suspension cultures of cotton (Gossypium hirsutum L.) |
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Authors: | John J. Finer |
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Affiliation: | (1) CIBA-GEIGY Corporation, 27709 Research Triangle Park, NC, USA;(2) Present address: Department of Agronomy, Ohio Agricultural Research and Development Center, The Ohio State University, 44691 Wooster, OH, USA |
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Abstract: | Maintainable, highly embryogenic suspension cultures of cotton (Gossypium hirsutum L. cv. Coker 310 ) have been obtained. Callus cultures were initiated from cotyledonary tissues from aseptically-germinated seedlings. To establish the suspension cultures, callus tissue was placed in a liquid medium containing either 0.5 mg/l picloram or 0.1 mg/l 2,4-dichlorophenoxyacetic acid. For proliferation of the embryogenic suspension, 5 mg/l of 2,4-dichlorophenoxyacetic acid was used. Embryo development took place when the embryogenic tissue was transferred to an auxin-free liquid medium containing 15 mM glutamine. Early embryo development was fairly synchronous and large numbers of somatic embryos were produced. Regenerated plants were fertile and smaller than seed-derived plants.Abbreviations 2,4-D 2,4-dichlorophenoxyacetic acid - NAA 1-naphthaleneacetic acid - IAA indole-3-acetic acid |
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