Generation of electric current by chromatophores of Rhodospirillum rubrum and reconstitution of electrogenic function in subchromatophore pigment-protein complexes |
| |
Authors: | L.A. Drachev V.N. Frolov A.D. Kaulen A.A. Kondrashin V.D. Samuilov A.Yu. Semenov V.P. Skulachev |
| |
Affiliation: | Department of Bioenergetics, Laboratory of Bioorganic Chemistry, and Department of Microbiology, Biological Faculty, Moscow State University, Moscow 117234, U.S.S.R. |
| |
Abstract: | Lipoprotein complexes, containing (1) bacteriochlorophyll reaction centers, (2) bacteriochlorophyll light-harvesting antenna or (3) both reaction centers and antenna, have been isolated from chromatophores of non-sulphur purple bacteria Rhodospirillum rubrum by detergent treatments. The method of reconstituting the proteoliposomes containing these complexes is described. Being associated with planar azolectin membrane, proteoliposomes as well as intact chromatophores were found to generate a light-dependent transmembrane electric potential difference measured by Ag/AgCl electrodes and voltmeter. The direction of the electric field in proteoliposomes can be regulated by the addition of antenna complexes to the reconstitution mixture. The reaction center complex proteoliposomes generate an electric field of a direction opposite to that in chromatophores, whereas proteoliposomes containing reaction center complexes and a sufficient amount of antenna complexes produce a potential difference as in chromatophores. ATP and inorganic pyrophosphate, besides light, were shown to be usable as energy sources for electric generation in chromatophores associated with planar membrane. |
| |
Keywords: | CCCP 2,4,6-trichlorocarbonylcyanide phenylhydrazone inorganic pyrophosphate TMPD |
本文献已被 ScienceDirect 等数据库收录! |
|