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Culture conditions for enhanced cellulase production by a native strain of Penicillium purpurogenum
Authors:J. Steiner  C. Socha  J. Eyzaguirre
Abstract:A cellulolytic wild-type strain of Penicillium purpurogenum was isolated from a soil sample in southern Chile. It grew best at 28°C from an inoculum of 4×107 spores/100 ml medium. Highest endoglucanase activity was with Sigmacell as carbon source and corn steep liquor as nitrogen source. Wheat bran enhanced the production of endoglucanase and beta-glucosidase. The enzymes in the crude supernatants were stable up to 50°C and between pH 4.4 and 5.6 for 48 h.J.Steiner and C. Socha are with the Facultad de Ciencias Quimicas y Farmacéuticas, Universidad de Chile, Casilla 233, Santiago 1, Chile; J. Eyzaguirre is with the Laboratorio de Bioquímica, Pontificia Universidad Católica de Chile, Casilla 114-D, Santiago, Chile.
Keywords:Cellulase  enzyme production    /content/u220147481686411/xxlarge946.gif"   alt="  beta"   align="  MIDDLE"   BORDER="  0"  >-glucosidase  Penicillium purpurogenum  xylanases
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