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COMPARISON OF CHITIN STRUCTURES DERIVED FROM THREE COMMON WASP SPECIES (Vespa crabro LINNAEUS, 1758, Vespa orientalis LINNAEUS, 1771 and Vespula germanica (FABRICIUS, 1793))
Authors:Murat Kaya  Nil Ba?r?aç?k  Osman Seyyar  Talat Baran
Institution:1. Department of Biotechnology and Molecular Biology, Faculty of Arts and Sciences, Aksaray University, Aksaray, Turkey;2. Science and Technology Application and Research Center, Aksaray University, Aksaray, Turkey;3. Department of Biology, Science and Arts Faculty, Ni?de University, Ni?de, Turkey;4. Department of Chemistry, Faculty of Science and Letters, Aksaray University, Aksaray, Turkey
Abstract:There has been no study on the chitin structure of wasp species. Here, we selected the three most common wasp species belonging to the family Vespidae for chitin extraction and characterization. Chitin was isolated from each wasp species and characterized by Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X‐ray diffractometry (XRD), elemental analysis (EA), and scanning electron microscopy (SEM). The chitin contents of Vespa crabro, Vespa orientalis, and Vespula germanica were 8.3, 6.4, and 11.9%, respectively. The crystalline index (CrI) values for the chitin extracted from each species were 69.88, 53.92, and 50%, respectively. The most important finding of the study is that although the same method was used to extract chitin from each of the three wasp species, the degree of acetylation was different: for V. crabro and V. orientalis it was 96.85 and 99.82% (the chitin was extremely pure), respectively, whereas that for V. germanica the chitin was 79.83%.
Keywords:insect  wasp  Vespidae  chitin  characterization
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