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| 灵芝栽培菌株酯酶同工酶的酶谱多样性 | |
| 引用本文： | 隋玉龙,宋慧,杜金,牛姝力,李长田. 灵芝栽培菌株酯酶同工酶的酶谱多样性[J]. 菌物研究, 2013, 11(2): 136-140 |
| 作者姓名： | 隋玉龙 宋慧 杜金 牛姝力 李长田 |
| 作者单位： | 1. 吉林农业大学生命科学学院,长春,130118 2. 吉林农业大学生命科学学院,长春130118;吉林农业大学教育部食药用菌工程研究中心,长春130118 3. 吉林农业大学菌物研究所,长春,130118 |
| 基金项目： | 吉林省科技发展计划项目，长江学者和创新团队计划项目 |
| 摘 要： | 采用酯酶同工酶聚丙烯酰胺凝胶电泳(PAGE)方法,对灵芝属(GanodermaKarst.)9株灵芝进行品系间鉴定,并采用非加权组平均法(UPGMA)进行聚类分析。试验结果表明:在9个菌株(16 d)中共检测到40条酶带,各个菌株分别具有3至6条酶带,9个菌株共有4种酶谱类型。在相异系数为0.62时所有供试菌株归为1个群,在相异系数为0.81时,把9个菌株分为5个群。酯酶同工酶聚丙烯酰胺凝胶电泳鉴定方法可有效应用于灵芝属真菌品种鉴定及亲缘关系分析。 |
| 关 键 词： | 灵芝 酯酶同工酶 聚丙烯酰胺凝胶电泳 聚类分析 |
Esterase Isozyme Analysis of Ganoderma Strains |
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| SUl Yu-Iong,SONG Hui,DU Jin,NIU Shu-li,LI Chang-tian. Esterase Isozyme Analysis of Ganoderma Strains[J]. Journal of Fungal Research, 2013, 11(2): 136-140 | |
| Authors: | SUl Yu-Iong SONG Hui DU Jin NIU Shu-li LI Chang-tian |
| Affiliation: | 1. College of Life Sciences, Jilin Agricultural University, Changchun 130118, China ; 2. Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural Uni- versity, Changchun 130118, China; 3. Institute of Mycology, Jilin Agricultural University, Changchun , 130118, China) |
| Abstract: | To identify strains＇ genetic diversity and provide a basis for systematic taxonomy and breeding, 9 strains of Ganoderma were analyzed by using the technique of vertical slab poly-acrylamide gel elec- trophoresis. The results show that the 9 strains were divided into 5 groups at the dissimilarity coefficient 0.81 in the UPGMA tree. Strain 6 was the farest away from the others. The studies also show that identi- fication of esterase isozymes in polyacrylamide gel electrophoresis （PAGE） method can be effectively ap- plied to the fungal species identification and relationship research of Ganoderma. |
| Keywords: | Ganoderma esterase isozyme polyacrylamide gel electrophoresis cluster analysis |
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