New insight into the evolution of aquaporins from flowering plants and vertebrates: orthologous identification and functional transfer is possible |
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Authors: | Soto Gabriela Alleva Karina Amodeo Gabriela Muschietti Jorge Ayub Nicolás Daniel |
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Institution: | 1. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Avda. Rivadavia 1917. C1033AAJ Cuidad Autónoma de Buenos Aires, Argentina;2. Instituto de Genética “Ewald A. Favret”, CICVyA, INTA Castelar, De los Reseros S/N C25 (1712), Provincia de Buenos Aires, Argentina;3. Instituto de Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Vuelta de Obligado 2490 Piso 2, Buenos Aires, C1428ADN, Argentina;4. Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, C1428EGA, Argentina |
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Abstract: | Aquaporins (AQPs) represent a family of channel proteins that transport water and/or small solutes across cell membranes in the three domains of life. In all previous phylogenetic analysis of aquaporin, trees constructed using proteins with very low amino acid identity (<15%) were incongruent with rRNA data. In this work, restricting the evolutionary study of aquaporins to proteins with high amino acid identity (>25%), we showed congruence between AQPs and organismal trees. On the basis of this analysis, we defined 19 orthologous gene clusters in flowering plant species (3 PIP-like, 7 TIP-like, 6 NIP-like and 3 SIP-like). We described specific conserved motifs for each subfamily and each cluster, which were used to develop a method for automatic classification. Analysis of amino acid identity between orthologous monocotyledon and dicotyledon AQPs from each cluster, suggested that PIPs are under high evolutionary constraint. The phylogenetic analysis allowed us the assignment of orthologous aquaporins for very distant animal lineages (tetrapods-fishes). We also demonstrated that the location of all vertebrate AQPs in the ortholog clusters could be predicted by comparing their amino acid identity with human AQPs. We defined four AQP subfamilies in animals: AQP1-like, AQP8-like, AQP3-like and AQP11-like. Phylogenetic analysis showed that the four animal AQPs subfamilies are related with PIP-like, TIP-like, NIP-like and SIP-like subfamilies, respectively. Thus, this analysis would allow the prediction of individual AQPs function on the basis of orthologous genes from Arabidopsis thaliana and Homo sapiens. |
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Keywords: | AQPS aquaporins PIPs plasma membrane intrinsic proteins TIPs tonoplast intrinsic proteins NIPs NOD26-like intrinsic proteins SIPs small basic intrinsic proteins XIPs x intrinsic proteins HIPs hybrid intrinsic proteins GIPs GlpF-like intrinsic proteins PhaA thiolase II PhaB reductase PhaC polyhydroxybutyrate polymerase AACT acetoacetyl-CoA thiolase NJ neighbor-joining ME minimum evolution MP maximum parsimony ER endoplasmic reticulum PM plasma membrane |
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