| 花榈木组培苗茎段低温胁迫培养及耐冷性诱导 |
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| 引用本文： | 高丽,杨波,李洪林.花榈木组培苗茎段低温胁迫培养及耐冷性诱导[J].亚热带植物科学,2009,38(2):19. |
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| 作者姓名： | 高丽 杨波 李洪林 |
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| 作者单位： | 中国科学院 武汉植物园, 湖北 武汉 430074 |
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| 基金项目： | 中国科学院知识创新工程重要方向项目 |
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| 摘 要： | 以花榈木组培苗茎段为试材,在低温胁迫条件下进行耐冷性愈伤组织诱导培养。结果表明,5℃是花榈木茎段低温胁迫培养及耐冷性诱导的最适温度。经过5℃低温胁迫培养获得的耐冷性愈伤组织在分化培养基MS+6-BA 2.0 mg/L+NAA 0.25 mg/L上的分化率达70.0%,且再生植株的低温伤害率明显低于对照。
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| 关 键 词： | 花榈木 低温胁迫 耐冷性 |
| 收稿时间： | 2008-12-03 |
Stems Culture under Low Temperature Stress and Chilling Tolerance Induction of Ormosia henryi Seedlings Cultured in vitro |
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| GAO Li,YANG Bo,LI Hong-lin.Stems Culture under Low Temperature Stress and Chilling Tolerance Induction of Ormosia henryi Seedlings Cultured in vitro[J].Subtropical Plant Science,2009,38(2):19. |
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| Authors: | GAO Li YANG Bo LI Hong-lin |
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| Institution: | Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, Hubei China |
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| Abstract: | Stems of tissue culture seedlings of Ormosia henryi were cultured under low temperature stress. The results showed that the optimal temperature for induction of chilling tolerance callus was 5℃. The differentiation frequency of chilling tolerance callus cultured under 5℃ was 70.0% on MS solid medium supplemented with 6-BA 2.0 mg/L and NAA 0.25 mg/L, and the injury rate of regenerated plants from chilling tolerant callus was much lower than that of control group. |
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| Keywords: | Ormosia henryi low temperature stress chilling tolerance |
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