肺癌脑转移型A549/GFP-2 细胞的筛选及其条件液对脑微血管内皮细胞的作用

目的：建立肺癌脑转移模型，筛选脑转移倾向细胞A549/GFP-2，探讨A549 和A549/GFP-2 条件液对脑微血管内皮细胞的作用，揭示肺癌脑转移的机制。方法：利用胸腔原位注射法筛选出A549 脑转移细胞亚型A549/GFP-2，原代培养大鼠脑微血管内皮细胞，观察A549和A549/GFP-2 细胞条件液对脑微血管内皮细胞增殖的影响和细胞内HIF-1琢和VEGF表达的改变。结果：胸腔内原位种植较好地反应了临床肺癌脑转移的过程。不同浓度的A549 和A549/GFP-2 细胞条件液对脑微血管内皮细胞增殖的影响不同，低浓度（＜ 30%）对脑微血管内皮细胞有促进的作用；高浓度（＞ 60%）对脑微血管内皮细胞的增殖有不同程度的抑制作用，且有随浓度增加抑制作用增强的趋势。A549 和A549/GFP-2 细胞条件液能提高脑微血管内皮细胞内HIF-1alpha和VEGF 的表达。结论：胸腔内原位种植是建立肺癌脑转移的稳定模型。肺癌脑转移与肺癌细胞在生长过程中分泌的HIF-1alpha和VEGF等细胞因子破坏了脑微血管内皮细胞的结构有关。

The Selection of A549/GFP-2 of Brain Metastases FromLung and the Effectof Condition Mediumon Brain Microvascular Endothelial Cells

Objective:The model of brain metastases was established from lung, and the effect of A549 and A549/GFP-2 conditionmedium was explored on Cerebral Microvascular Endothelial Cells (CMEC). So the mechanism of brain metastases from lung wasindicted.Methods:A549/GFP-2 cells have been selected from A549 cells by orthotopic implantation in chest, and primary cultured ratcerebral microvascular endothelial cell. The proliferation have been observed in CMEC and the expression of HIF-1alpha and VEGFhave been evaluated in CMEC.Results:The orthotopic implantation of A549 was the bettest way for brain metastases of lung cancer inchest. Low concentration (<30%) could promote proliferation of CMEC, but high concentrations (>60%) could inhibit proliferation ofCMEC. And the effect of inhibition enhanced with increasing concentration. The conditioned medium of A549 and A549/GFP-2 cellscould increase the expression of HIF-1alpha and VEGF in CMEC.Conclusion:Orthotopic implantation in chest is the stable model forbrain metastases fromlung. The brain metastasis fromlung cancer relates to destruction of structure of CMEC.