Variation in GUS activity in vegetatively propagated Hevea brasiliensis transgenic plants |
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Authors: | Ludovic Lardet Julie Leclercq Elise B��nistan Florence Dessailly G��rald Oliver Florence Martin Pascal Montoro |
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Institution: | (1) CIRAD, UMR AGAP, 34398 Montpellier, France; |
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Abstract: | Hevea brasiliensis transgenic plants are regenerated from transgenic callus lines by somatic embryogenesis. Somatic embryogenesis is not yet
available for commercial propagation of Hevea clones, which requires conventional grafting of buds on rootstock seedlings (budding). The stability of transgene expression
in budded plants is therefore necessary for further development of genetic engineering in rubber trees. Transgene expression
was assessed by fluorimetric beta-glucuronidase (GUS) activity in fully developed leaves of in vitro plants from transgenic
lines and their sub-lines obtained by budding. A large variation in GUS activity was found in self-rooted in vitro plants
of five transgenic lines, and the absence of activity in one line suggested transgene silencing. Beyond confirming transmissibility
of the reporter gene by budding and long-term expression, a quantification of GUS activity revealed that greater variability
existed in budded plants compared to self-rooted mother in vitro plants for three transgenic lines. Although somatic embryogenesis
provided more stable GUS activity, budding remained an efficient way of propagating transgenic plants but transgene expression
in budded plants should be verified for functional analysis and further development. |
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