Oxidation of nitroxide radicals by the reaction of hemoglobin with hydrogen peroxide |
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Authors: | T Yamaguchi T Nakano E Kimoto |
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Institution: | Department of Chemistry, Faculty of Science, Fukuoka University, Jonan-ku, Fukuoka 814-01, Japan |
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Abstract: | The ESR signal of 4-hydroxy-1-oxyl-2,2,6,6-tetramethylpiperidine in hemoglobin solution decreased drastically by the addition of hydrogen peroxide. The results of ion-exchange chromatography and sodium tetraphenylborate on the reaction solution showed an oxidation of the nitroxide radical to cation form. On the basis of the comparison of thin layer-chromatogram with the reaction products of the nitroxide radicals with HCl or Br2, the formation of 4-hydroxy-1-oxo-2,2,6,6- tetramethylpiperidinium cation was demonstrated. This result was supported by the 13C NMR measurement. |
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Keywords: | HOTMP 4-hydroxy-l-oxyl-2 2 6 6-tetramethylpiperidine TLC thin-layer chromatography |
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