The conserved role of the AKT/GSK3 axis in cell survival and glycogen metabolism in Rhipicephalus (Boophilus) microplus embryo tick cell line BME26期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

*The conserved role of the AKT/GSK3 axis in cell survival and glycogen metabolism in Rhipicephalus* (Boophilus) microplus embryo tick cell line BME26**

Authors:	Leonardo Araujo de Abreu,Christiano Calixto,Camila Fernanda Waltero,Bá rbara Pitta Della Noce,Naftaly Wang'ombe Githaka,Adriana Seixas,Luí s Fernando Parizi,Satoru Konnai,Itabajara da Silva Vaz Jr.,Kazuhiko Ohashi,Carlos Logullo

Affiliation:	1. LQFPP, CBB and Unidade de Experimentação Animal, RJ, UENF, Avenida Alberto Lamego, 2000, Horto, CEP 28013-602, Campos dos Goytacazes, RJ, Brazil;2. Centro de Biotecnologia and Faculdade de Veterinária, UFRGS, C.P. 15005, Av. Bento Gonçalves 9500, Prédio 43421, Campus do Vale, CEP 91501-970, Porto Alegre RS, Brazil;3. Laboratory of Infectious Diseases, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 9, Kita-ku Sapporo, 060-0818, Japan;4. Universidade Federal de Ciências da Saúde de Porto Alegre, R. Sarmento Leite, 245, CEP 90050-170, Porto Alegre, RS, Brazil

Abstract:

Keywords:	10-DEBC, 10-(4&prime -(N,N-Diethylamino) butyl)-2-chlorophenoxazine hydrochloride AKT, Protein kinase B CN, Negative control DMSO, Dimethyl sulfoxide dsRNA, Double-stranded RNA dsCN, Unrelated dsRNA dsAKT, Double-stranded RNA for protein kinase B dsGSK, Double-stranded RNA for glycogen synthase kinase 3 FBS, Fetal bovine serum GSK3, Glycogen synthase kinase 3 IC₅₀, Inhibitory concentration to reduce cell viability by 50% ISP, Insulin/insulin-like growth factor signaling pathway IGF, Insulin-like growth factor MTT, 3-(4,5 Dymethil-2-thiazolyn)-2-5-dyphenyl-2H-tetrazolium bromide) ORF, Open reading frame PI3K, Phosphatidylinositol 3-OH kinase RTK, Receptor tyrosine kinase RNAi, Ribonucleotic acid interference RACE, Rapid amplification of cDNA ends RmAKT, Rhipicephalus microplus protein kinase B
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