Zinc induced folding is essential for TIM15 activity as an mtHsp70 chaperone |
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Authors: | Hugo Fraga,Elena Papaleo,Sonia Vega,Adriá n Velazquez-Campoy,Salvador Ventura |
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Affiliation: | 1. Departament de Bioquímica i Biologia Molecular, Facultat de Biociències, Universitat Autònoma de Barcelona, E-08193 Bellaterra Spain;2. Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, E-08193 Bellaterra Spain;3. Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milan, Italy;4. Institute of Biocomputation and Physics of Complex Systems, Unidad Asociada IQFR-CSIC-BIFI, Universidad de Zaragoza, Zaragoza, Spain;5. Fundación ARAID, Diputación General de Aragón, Spain |
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Abstract: | BackgroundTIM15/Zim17 in yeast and its mammalian ortholog Hep are Zn2 + finger (Cys4) proteins that assist mtHsp70 in protein import into the mitochondrial matrix.MethodsHere we characterized the Zn2 + induced TIM15 folding integrating biophysical and computational approaches.ResultsTIM15 folding occurs from an essentially unstructured conformation to a Zn2 +-coordinated protein in a fast and markedly temperature-dependent process. Moreover, we demonstrate unambiguously that Zn2 + induced TIM15 folding is essential for its role as mtHsp70 chaperone since in the unstructured apo state TIM15 does not bind to mtHsp70 and is unable to prevent its aggregation. Molecular dynamics simulations help to understand the crucial role of Zn2 + in promoting a stable and functional 3D architecture in TIM15. It is shown that the metal ion, through its coordinating cysteine residues, can mediate relevant long-range effects with the interaction interface for mtHsp70 coupling thus folding and function.ConclusionsZn2 + induced TIM15 folding is essential for its function and likely occurs in mitochondrial matrix where high concentrations of Zn2 + were reported.General significanceThe combination of experimental and computational approaches presented here provide an integrated structural, kinetic and thermodynamic view of the folding of a mitochondrial zinc finger protein, which might be relevant to understand the organelle import of proteins sharing this fold. |
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Keywords: | AMS, (4-acetamido-49-maleimidylstibene-2,29-disulfonic acid) ANS, 8-anilino-1-naphthalenesulfonic acid DCCM, dynamical cross-correlation matrix DSC, differential scanning calorimetry DTT, dithiothreitol EDTA, ethylene diamine tetraacetic acid ITC, isothermal titration calorimetry MD, molecular dynamics PSN, protein structure network TCEP, tris(2-carboxyethyl)phosphine ThT, thioflavin T |
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