Unnatural amino acids as probes of protein structure and function |
| |
Authors: | Dougherty D A |
| |
Institution: | Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA. dad@igor.caltech.edu |
| |
Abstract: | Nonsense suppression methodology, for incorporating unnatural amino acids into proteins, has enabled a wide range of studies into protein structure and function using both in vitro and in vivo translation systems. Although methodological challenges remain, scores of unnatural amino acids have been employed that include both subtle and dramatic variants of the natural set. A number of insights that would not have been possible using conventional site-directed mutagenesis have been gained. |
| |
Keywords: | Unnatural amino acids protein structure protein function nonsense suppression biophysical probes backbone mutations |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|