Functional Expression of Agrobacterium tumefaciens T-DNA onc-genes in Asparagus Crown Gall Tissues |
| |
Authors: | Prinsen Els; Bytebier Benny; Hernalsteens Jean-Pierre; De Greef Jan; Van Onckelen Henri |
| |
Institution: | 1Department of Biology, UIA Universiteitsplein I, B-2610 Wilrijk, Belgium
2Laboratorium voor Genetische Virologie, VUB Paardestraat 65 B-1640 St. Genesius-Rode, Belgium |
| |
Abstract: | We analyzed Asparagus crown gall tissues transformed with A.tumefaciens C58 and C58C1 (pTiB6S3) and selected for hormoneautotrophic growth. No increased IAA levels were observed inthe Asparagus tumor lines notwithstanding the presence of allthree T-DNA onc genes. The endogenous cytokinin levels indicatethat Asparagus crown gall is dependent on enhanced zeatin ribosideequivalent levels for its growth. We conclude that phytohormone autotrophic growth of Asparaguscrown gall tissue seems only to be dependent upon an activegene 4, inducing enhanced cytokinin levels. Moreover, the presenceof an active gene 1 seems to be lethal as was indicated by theabsence of tryptophan-2-mono-oxygenase activity in transformedtissues and the toxicity of exogenously supplied indole-3-acetamide(IAM) or naphthalene-1-acetamide (NAM) as a substitute for anactive gene 1. (Received August 7, 1989; Accepted October 31, 1989) |
| |
Keywords: | |
本文献已被 Oxford 等数据库收录! |
|