Analysis of age-associated alteration in the synthesis of HMG nonhistone proteins of the rat liver |
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Authors: | M K Thakur S Prasad |
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Institution: | (1) Biochemistry and Molecular Biology Laboratory, Centre of Advanced Study in Zoology, Banaras Hindu University, 221 005 Varanasi, India |
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Abstract: | HMG proteins were extracted with 5% PCA or 0.35 M NaCl from whole tissue, nuclei or chromatin of the liver of young (19 weeks) and old (118 weeks) male rats. They were resolved on acetic acid-urea polyacrylamide gel. The electrophoretic patterns of the major HMG proteins 1, 2, 14 and 17 of both ages are similar. The in vitro synthesis of HMG 1 and 2 decreases, but that of HMG 14 and 17 increases considerably in the liver of old rats. The synthesis of different HMG proteins is modulated differentially by spermine, butyrate, dexamethasone and 3-aminobenzamide in the liver of young and old rats. These findings suggest that HMG proteins contribute to alterations in the organization of chromatin and expression of genes during aging. |
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Keywords: | aging HMG proteins modulators synthesis |
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