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Diversity of culturable <Emphasis Type="Italic">Streptomycetes</Emphasis> from wheat cropping system of fertile regions of Indo-Gangetic Plains,India
Authors:Nityanand Malviya  Arvind K Yadav  Mahesh S Yandigeri  Dilip K Arora
Institution:(1) National Bureau of Agriculturally Important Microorganisms, Kusmaur, Kaithauli, Mau Nath Bhanjan, 275101, India;
Abstract:Streptomycetes is one of the most important genera, representing up to 70% population among all soil actinomycetes. We investigated the genotypic diversity of culturable Streptomycetes, freshly isolated from thirty-eight different soil samples (wheat cropping system) from Indo-Gangetic Plains of India. A total of 238 morphologically distinct actinomycetes colonies were isolated and clustered together by restriction fragment length polymorphism analysis of 16S rRNA gene. Identification of representative isolates from each cluster was carried out by micro/macroscopic analysis as well as biochemical characterization, and further confirmed by 16S rRNA sequencing (sequences were submitted in public database, NCBI).
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