The development of a new adjuvant lipid-saponin complex and its use at experimental immunization by bacterial antigen |
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| Authors: | A. V. Tsybulsky N. M. Sanina I. A. Lee A. M. Popov E. Ya. Kostetsky O. Yu. Portnyagina V. L. Shnyrov |
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| Affiliation: | (1) Department of Biochemistry and Biotechnology, Far Eastern State University, ul. Oktyabrskaya 27, Vladivostok, 690950, Russia;(2) Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, pr. 100 let Vladivostoku 159, Vladivostok, 690022, Russia;(3) Departamento de Bioquimica y Biologia Molecular, Universidad de Salamanca, s/n 37007, Spain |
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| Abstract: | Immunostimulating complexes (ISCOM) have been modified by replacement of structural components of ISCOM; phosphatidylcholine has been replaced, by glycolipid of monogalactosyldiacylglycerol from sea macrophytes, saponin QuilA has been replaced by triterpene glycoside, Cucumarioside A2-2. The resultant complex includes morphological structures of two types: the ISCOM-like structures with the characteristic morphology and sizes and also the tubular structures with diameter of about 40 nm and length of 150–400 nm. We have defined these structures as TI-complexes. These TI-complexes exhibit considerably lower toxicity than ISCOM. They may include an amphiphilic protein antigen and provide immunoadjuvant effect during experimental vaccination. Under conditions of the experimental immunization of mice with a weak immunogen (subunit membrane pore protein from Y. pseudotuberculosis), TI-complexes with antigen provided stronger humoral immune response to antigen than the complexes of porin with classical ISCOM, liposomes and Freund’s adjuvant. Thus, the TI-complexes represent a new type of adjuvant carriers for antigens. |
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| Keywords: | adjuvant ISCOM triterpene glycoside monogalactosyldiacylglycerol (MGDG) vaccine porin |
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