Messenger RNA properties of primary cultures of thyroid cells isolated from normal thyroid tissue and from patients with various thyroid diseases.

When compared to cells isolated from normal thyroid tissue, cells isolated from colloid adenoma have the same total polyadenylic acid content and total template activity. However, in both the cells isolated from diffuse non toxic goiter and from toxic adenoma, these two values are increased, the most striking effect being observed in the latter case. Moreover, as compared to normal thyroid tissue, in the three thyroid diseases and particularly in toxic adenoma, we observed an increase in the polyadenylic acid and messenger activity associated to RNA sedimenting at greater than 30 S, which correspond probably to thyroglobulin messenger RNA.