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Separation of human endothelial cells from fibroblasts by centrifugation in Percoll gradients |
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| Authors: | Rosella Sbarbati |
| Affiliation: | (1) C.N.R. Institute of Clinical Physiology, Via Savi 8, 56100 Pisa, Italy |
| Abstract: | Human endothelial cells from the umbilical vein and skin fibroblasts can be separated by means of centrifugation in a density gradient of Percoll. Cells show a good recovery in culture. Viability is not impaired. |
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