Kinetic analysis of inhibition of cAMP-dependent protein kinase catalytic subunit by the peptide-nucleoside conjugate AdcAhxArg6 |
| |
Authors: | Kuznetsov Aleksei Uri Asko Raidaru Gerda Järv Jaak |
| |
Affiliation: | Institute of Organic and Bioorganic Chemistry, University of Tartu, 2 Jakobi Str, 51014, Estonia. |
| |
Abstract: | Kinetic analysis of the inhibition of the phosphorylation of Kemptide, (LRRASLG), catalyzed by the catalytic subunit of cAMP-dependent protein kinase, by a peptide-nucleoside conjugate inhibitor AdcAhxArg6 was carried out over a wide range of ATP and peptide concentrations. A simple procedure was proposed for characterization of the interaction of this inhibitor with the free enzyme, and with the enzyme-ATP and enzyme-peptide complexes. The second-order rate constants, calculated from the steady-state reaction kinetics, were used for this analysis to avoid the complications related to the complex catalytic mechanism of the protein kinase catalyzed reaction. |
| |
Keywords: | cAMP-dependent protein kinase Bisubstrate analog inhibitor Peptide phosphorylation |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|