| 激光诱变微生物的遗传和刺激效应机理及育种研究 Ⅰ.CO_2激光对酒精酵母菌的诱变效应 |
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| 引用本文： | 陈有为,李绍兰,杨丽源,张玲琪,魏蓉城,伏云昌,周凌云.激光诱变微生物的遗传和刺激效应机理及育种研究 Ⅰ.CO_2激光对酒精酵母菌的诱变效应[J].激光生物学报,1996(1). |
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| 作者姓名： | 陈有为 李绍兰 杨丽源 张玲琪 魏蓉城 伏云昌 周凌云 |
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| 作者单位： | 云南大学省微生物研究所!昆明，650091，云南大学省微生物研究所!昆明，650091，云南大学省微生物研究所!昆明，650091，云南大学生物系!昆明，650091，云南大学省微生物研究所!昆明，650091，昆明理工大学非线性研究中心!昆明，650093，昆明理工大学非线性研究中心!昆明，650093 |
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| 基金项目： | 国家自然科学基金!39460025，云南省应用基础研究基金!39460036 |
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| 摘 要： | 本文选择甘蔗糖蜜酒精工业性生产用酿酒酵母菌SaccharomycescerevisiaeAS2.1189作为出发菌株，利用CO2激光对其进行辐照处理．制定出该菌最佳致死量的基础上，从A、B……E等5个不同照射剂量中，共挑取辐照菌639株．经糖蜜发酵后，对产乙醇含量进行了气相色谱分析．结果筛选出乙醇含量高于出发菌株5％～11％范围的辐照菌有5株；而低于10％～33％之间的为9株．表明CO2激光对该酵母菌产乙醇含量的高低有刺激效应．亦提示了CO2激光对酵母菌乙醇脱氢酶系的激活有刺激作用．
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| 关 键 词： | CO_2激光 酵母菌 乙醇 |
A FUNCTION OF MUTAGENESIS ON SACCHAROMYCES CEREVISIAE BY CO_2 LASER |
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| Chen Youwei,Li Shaolan,Yang Liyuan,Zhang Lingqi,Wei Rongcheng,Fu Yunchang and Zhou Lingyun.A FUNCTION OF MUTAGENESIS ON SACCHAROMYCES CEREVISIAE BY CO_2 LASER[J].ACTA Laser Biology Sinica,1996(1). |
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| Authors: | Chen Youwei Li Shaolan Yang Liyuan Zhang Lingqi Wei Rongcheng Fu Yunchang and Zhou Lingyun |
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| Abstract: | An effect of stimulation on the production of the ethanol from yeasts was illustrated by utilizing CO2 laser. And 639 irradiated strains was selected to produce the alcohol from molasses in Gas Chromatography. The conditional experiments of 5 different irradiation dosages were studied in the meantime. The results indicated on the percentage of the ethanol from 5 variational strains could increase 5 to 11 and the other 9 variational strains decrease 10 to 33 under basis of Saccharomyces cerevisiae AS 2. 1189. In conclusion,the mutagenic action of CO2 laser infered actually that the physiological stimulation on cells of yeast,including the dehydrogenase of ethanol and perhaps the resequences of base paire in DNA or RNA. |
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| Keywords: | CO_2 laser Yeast The ethanol |
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