Indole-3-acetaldehyde oxidase of pea seedlings |
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| Authors: | Susumu Miyata Yonezo Suzuki Seiichiro Kamisaka Yoshio Masuda |
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| Institution: | Research Institute for Biochemical Regulation, School of Agriculture, Nagoya University, Chikusa-ku, Nagoya 464, Japan;Department of Biology, Faculty of Science, Osaka City University, Sumiyoshi-ku, Osaka 558, Japan. |
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| Abstract: | Indole-3-acetaldehyde oxidase was partially purified from the epicotyl of Pisum satiyum seedlings by column chromatography using CM-Sephadex and Sephadex G-150. The enzyme was only active in the presence of molecular oxygen. The activity was maximal at pH 8.0, and the Km value for indole-3-acetaldehyde was 1.4 × 10−3 M . The enzyme was inhibited strongly by p -hydroxymercuribenzoate, cyanide and hydroxylamine, suggesting that it contains sulfhydryl group(s) and a metal component such as iron. |
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| Keywords: | Indole-3-acetaldehyde oxidase Pisum sativum pea epicotyls |
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