Improved gel-filtration method for analysis of estrogen receptor binding |
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| Authors: | V C Godefroi S C Brooks |
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| Affiliation: | Detroit Institute of Cancer Research, Division of the Michigan Cancer Foundation and Department of Biochemistry, Wayne State University School of Medicine, Detroit, Michigan 48201 USA |
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| Abstract: | A method has been described whereby 100- to 300-mg amounts of breast tumor tissue can be analyzed for cytoplasmic estrogen receptor binding capacity. This procedure differentiates specific from nonspecific estrogen binding. In addition, competitive adsorption of estrogen to Sephadex gel has been measured and true receptor binding capacities evaluated. By submitting the corrected receptor binding data to Scatchard plot analysis, the dissociation constant of the estrogen receptor complex was determined with greater accuracy. |
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