EB1 reveals mobile microtubule nucleation sites in Arabidopsis |
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Authors: | Chan Jordi Calder Grant M Doonan John H Lloyd Clive W |
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Affiliation: | Department of Cell and Developmental Biology, John Innes Centre, Colney, Norwich, NR4 7UH, UK. |
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Abstract: | In plants, it is unclear how dispersed cortical microtubules are nucleated, polarized and organized in the absence of centrosomes. In Arabidopsis thaliana cells, expression of a fusion between the microtubule-end-binding protein AtEB1a and green fluorescent protein (GFP) results in labelling of spindle poles, where minus ends gather. During interphase, AtEB1a-GFP labels the microtubule plus end as a comet, but also marks the minus end as a site from which microtubules can grow and shrink. These minus-end nucleation sites are mobile, explaining how the cortical array can redistribute during the cell cycle and supporting the idea of a flexible centrosome in plants. |
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