Effect of additives on the esterification activity of immobilized Candida antarctica lipase |
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Authors: | Dasciana S Rodrigues George P Cavalcante Giovanilton F Silva Andrea L O Ferreira Luciana R B Gonçalves |
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Institution: | 1. Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, Bloco 709, Fortaleza, CE, CEP: 60455-760, Brazil 2. Tecnologias Bioenergéticas – TECBIO, R. R?mulo Proen?a, S/N – Campus do Pici, Fortaleza, CE, 60455-760, Brazil
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Abstract: | In this work, the stabilizing effect of bovine serum albumin (BSA), peptone (PEP), and polyethylene glycol (PEG) during immobilization
of Candida antarctica lipase on activated carbon was investigated. The influence of enzyme concentration and type of additive, added during the
immobilization procedure, was studied using a 22 factorial central composite design. The goal was to maximize the synthetic activity of butyl butyrate, using butyric acid
and butanol as substrate in n-heptane. An increase of 31–58% in the esterification activity was obtained when enzyme concentration on the supernatant was
enhanced from 86.50 U m L−1 to 226.80 U mL−1. An enhancement in esterification activity of 38–68.95% was observed, depending on the initial enzyme concentration, when
PEP was used instead of BSA. No significant increase in the esterification activity was observed when PEP was replaced by
PEG. However, thermal stability tests at 50 °C showed that PEG had a higher stabilizing effect. |
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Keywords: | Candida antarctica lipase B Enzyme immobilization Activated carbon Adsorption and additives |
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