Cloning and characterization of two cytoplasmic dynein intermediate chain genes in mouse and human |
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Authors: | Crackower M A Sinasac D S Xia J Motoyama J Prochazka M Rommens J M Scherer S W Tsui L C |
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Affiliation: | Department of Molecular and Medical Genetics, University of Toronto, The Hospital for Sick Children, Ontario, Canada. |
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Abstract: | Cytoplasmic dynein is a large multisubunit microtubule-based motor protein, which mediates movement of numerous intracellular organelles. We report here the identification of the human homologue of cytoplasmic dynein intermediate chain 1 gene (DNCI1) located on human chromosome 7q21.3-q22.1. The mouse orthologue (Dnci1) was identified along with another highly related gene, Dnci2, and their RNA in situ expression patterns were examined during mouse embryogenesis. Dnci1 was found to have a highly restricted expression domain in the developing forebrain as well as the peripheral nervous system (PNS), while Dnci2 displayed a broad expression profile throughout the entire central nervous system and most of the PNS. A dynamic expression profile was also found for Dnci2 in the developing mouse limb bud. The data presented here provide a framework for the further analysis of the functional role of Dnci1 and Dnci2 in mouse and DNCI1 in human. |
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