In vitro and in vivo antimicrobial activity of two alpha-helical cathelicidin peptides and of their synthetic analogs |
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Authors: | Benincasa Monica Skerlavaj Barbara Gennaro Renato Pellegrini Antonio Zanetti Margherita |
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Institution: | Department of Biochemistry, Biophysics and Macromolecular Chemistry, University of Trieste, Via Giorgieri, 1, I-34127 Trieste, Italy. zanetti@icgeb.trieste.it |
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Abstract: | Two alpha-helical antimicrobial peptides (BMAP-27 and -28) and four synthetic analogs were compared for in vitro and in vivo antimicrobial efficacy. All peptides proved active in vitro at micromolar concentrations against a range of clinical isolates, including antibiotic-resistant strains. BMAP-27 and two analogs were more effective towards Gram-negative, and BMAP-28 towards Gram-positive organisms. In addition, BMAP-28 provided some protection in vitro against human herpes simplex virus type 1 (HSV-1). The parent peptides and mBMAP-28 analog protected mice from lethal i.p. infections in an acute peritonitis model at peptide doses significantly lower than those toxic to the animals, suggesting a satisfactory therapeutic index. |
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