Synovial mesenchymal stem cells accelerate early remodeling of tendon-bone healing |
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Authors: | Young-Jin Ju Takeshi Muneta Hideya Yoshimura Hideyuki Koga Ichiro Sekiya |
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Institution: | (1) Section of Orthopedic Surgery, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan;(2) Center of Excellence Program for Frontier Research on Molecular Destruction and Reconstruction of Tooth and Bone, Tokyo Medical and Dental University, Tokyo, Japan;(3) Section of Cartilage Regeneration, Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113–8519, Japan |
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Abstract: | Tendon-bone healing is important for the successful reconstruction of the anterior cruciate ligament by using the hamstring
tendon. Mesenchymal stem cells (MSCs) have attracted much interest because of their self-renewing potential and multipotentiality
for possible clinical use. We previously reported that MSCs derived from synovium had a higher proliferation and differentiation
potential than the other MSCs that we examined. The purpose of this study was to investigate the effect and mechanism of the
implantation of the synovial MSCs on tendon-bone healing in rats. Half of the Achilles’ tendon grafts of rats were inserted
into a bone tunnel from the tibial plateau to the tibial tuberosity with a suture-post fixation. The bone tunnel was filled
with MSCs labeled with fluorescent marker DiI or without MSCs as the control. The tendon-bone interface was analyzed histologically,
and collagen fibers were quantified. At 1 week, the tendon-bone interface was filled with abundant DiI-positive cells, and
the proportion of collagen fiber area was significantly higher in the MSC group than in the control group. By 2 weeks, the
proportion of oblique collagen fibers, which appeared to be Sharpey’s fibers, was significantly higher in the MSC group than
in the control group. At 4 weeks, the interface tissue disappeared, and the implanted tendon appeared to attach to the bone
directly in both groups. DiI-labeled cells could no longer be observed. Implantation of synovial MSCs into bone tunnel thus
accelerated early remodeling of tendon-bone healing, as shown histologically.
This study was supported in part by grants from the Japan Society for the Promotion of Science (19591752) and from the Center
of Excellence Program for Frontier Research on Molecular Destruction and Reconstruction of Tooth and Bone at Tokyo Medical
and Dental University to T.M. and from the Japan Society for the Promotion of Science (18591657) to I.S. |
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Keywords: | Mesenchymal stem cells Tendon-bone healing Anterior cruciate ligament reconstruction Cell therapy Synovium Rat (Sprague Dawley) |
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