基于16S rRNA基因测序分析微生物群落多样性

微生物群落多样性的研究对于挖掘微生物资源,探索微生物群落功能,阐明微生物群落与生境间的关系具有重要意义。随着宏基因组概念的提出以及测序技术的快速发展,16S rRNA基因测序在微生物群落多样性的研究中已被广泛应用。文中系统地介绍了16S rRNA基因测序分析流程中的四个重要环节,包括测序平台与扩增区的选择、测序数据预处理以及多样性分析方法,就其面临的问题与挑战进行了探讨并对未来的研究方向进行了展望,以期为微生物群落多样性相关研究提供参考。

Exploration of microbial diversity based on 16S rRNA gene sequence analysis

Surveying diversity of microbial communities has great significance for exploiting microbial resource, exploring functions of microbial communities, elucidating relations between microbial communities and their habitat. With the proposal of the concept of "metagenomics" and the development of sequencing technology, 16S rRNA gene profiling has been widely applied in the survey of microbial diversity. This review introduces four important stages of 16S rRNA gene sequencing analysis, including selection of sequencing platforms and hypervariable regions, sequencing data preprocess and methods of diversity analysis. Furthermore, the current challenges and future prospects to 16S rRNA gene profiling are discussed. This review aims to provide a reference for microbial diversity researches.