13C labeling experiments at metabolic nonstationary conditions: An exploratory study |
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| Authors: | Sebastian Aljoscha Wahl Katharina Nöh Wolfgang Wiechert |
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| Affiliation: | 1.Bioprocess Engineering,Max Planck Institute for Dynamics of Complex Technical Systems,Magdeburg,Germany;2.Fermentation Technology,Institute of Biotechnology 2, Research Centre Jülich GmbH,Jülich,Germany;3.Department of Simulation, Institute of Systems Engineering,University of Siegen,Siegen,Germany |
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| Abstract: | Background Stimulus Response Experiments to unravel the regulatory properties of metabolic networks are becoming more and more popular. However, their ability to determine enzyme kinetic parameters has proven to be limited with the presently available data. In metabolic flux analysis, the use of 13C labeled substrates together with isotopomer modeling solved the problem of underdetermined networks and increased the accuracy of flux estimations significantly. |
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