| 综述: 大鼠胚胎干细胞遗传操作技术的研究进展 |
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| 引用本文： | 孟姝.综述: 大鼠胚胎干细胞遗传操作技术的研究进展[J].生物化学与生物物理进展,2011,38(11):995-1000. |
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| 作者姓名： | 孟姝 |
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| 作者单位： | 中国科学院生物物理研究所，北京100101 |
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| 摘 要： | 大鼠胚胎干细胞(ES)的成功建立使大鼠的遗传学操作成为可能,运用同源重组原理改造ES细胞的基因,为建立时空特异性的基因敲除大鼠模型提供了基础.本文主要回顾了大鼠ES细胞的建立过程,总结了大鼠ES的培养、鉴定技术,分析了各种大鼠基因敲除技术的优劣势和未来前景.在干细胞研究蓬勃发展的背景下,作为最有效地定向修饰基因的技术手段,基于大鼠ES细胞的基因敲除技术将在揭示基因的生理功能、研究人类疾病的遗传机制以及寻找新药物靶标的过程中发挥更加重要的作用.
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| 关 键 词： | ES细胞 遗传操作 基因敲除 干细胞 |
| 收稿时间： | 2011/10/19 0:00:00 |
| 修稿时间： | 2011/10/19 0:00:00 |
Review: Progress in the genetic manipulation technology of Embryonic Stem Cells in Rats |
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| MENG Shu.Review: Progress in the genetic manipulation technology of Embryonic Stem Cells in Rats[J].Progress In Biochemistry and Biophysics,2011,38(11):995-1000. |
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| Authors: | MENG Shu |
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| Institution: | The Core Facility for Protein Research, Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101, China |
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| Abstract: | The successful establishment of rat ES cell lines enables possible genetic manipulation in rats. Using homologous recombination to modify ES cell genes provides a foundation to temporal and tissue-specific knockout rats. The author introduces the establishment process of rat ES cell, summarizes its culture and identification skills, and analyzes the weakness and strength of various rat knockout techniques. In the context of increasing research on stem cell, knockout technology based on rat ES cells, which allows the most efficient modification of specific genes, shall contribute more to elucidate gene function, dissect the genetic mechanisms of human diseases and identify potential targets for drug development. |
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| Keywords: | ES cell genetic manipulation gene knockout stem cell |
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