Ewing Sarcoma Cells Secrete EWS/Fli-1 Fusion mRNA via Microvesicles |
| |
| Authors: | Masanori Tsugita Nami Yamada Shunsuke Noguchi Kazunari Yamada Hiroshi Moritake Katsuji Shimizu Yukihiro Akao Takatoshi Ohno |
| |
| Affiliation: | 1. Department of Orthopaedic Surgery, Gifu University Graduate School of Medicine, Gifu, Gifu, Japan.; 2. United Graduate School of Drug Discovery and Medical Information Sciences, Gifu, Gifu, Japan.; 3. United Graduate School of Veterinary Sciences, Gifu University, Gifu, Gifu, Japan.; 4. Division of Pediatrics, Department of Reproductive and Developmental Medicine, Faculty of Medicine, University of Miyazaki, Miyazaki, Miyazaki, Japan.; University Hospital of Modena and Reggio Emilia, Italy, |
| |
| Abstract: | Tumours defined as Ewing sarcoma (ES) constitute a group of highly malignant neoplasms that most often affect children and young adults in the first 2 decades of life. The EWS/Fli-1 fusion gene, a product of the translocation t(11;22) (q24; 12), is detected in 95% of ES patients. Recently, it was validated that cells emit a heterogeneous mixture of vesicular, organelle-like structures (microvesicles, MVs) into their surroundings including blood and body fluids, and that these MVs contain a selected set of tumor-related proteins and high levels of mRNAs and miRNAs. In this present study, we detected the Ewing sarcoma-specific EWS/Fli-1 mRNA in MVs from the culture medium of ES cell lines carrying t(11;22) (q24; 12). Also, we detected this fusion gene in approximately 40% of the blood samples from mice inoculated with xenografts of TC135 or A673 cells. These findings indicate the EWS/Fli-1 mRNA in MVs might be a new non-invasive diagnostic marker for specific cases of Ewing sarcoma. |
| |
| Keywords: | |
|
|
