Triple Silver Impregnation for Selective Staining of Avian Nerves |
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Authors: | A B Gilbert |
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Institution: |
a Poultry Research Centre, King's Buildings, Edinburgh, Scotland |
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Abstract: | Frozen sections of avian tissue fixed 7 days or longer in 10% formalin or formol-saline are cut at 20-50 μ, left in distilled water for 2 hr, and placed in 0.002% aqueous AgNO3 for 3-4 days. Subsequent procedure is essentially that of Weddell and Glees. Sections are placed in 20% AgNO3 for 30 min, then carried through 3 baths of 3% formalin in less than 10 min. Immediately thereafter they are washed 1-2 sec in a 0.1% solution of NH4OH (cone) and placed in the ammoniacal silver solution (made with 20% AgNO3) until the nerves become distinct, as seen under a microscope; usually, in about 15 min. After washing briefly, the sections are fixed in 5% Na2S2O3 for 3-10 min, dehydrated, cleared, and mounted in the usual way. |
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