GABAA Receptors of Cerebellar Granule Cells in Culture: Interaction with Benzodiazepines |
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Authors: | Aroldo Cupello Mario Di Braccio Elena Gatta Giancarlo Grossi Periklis Nikas Francesca Pellistri Mauro Robello |
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Affiliation: | 1. Dipartimento di Fisica, Università di Genova, Via Dodecaneso 33, 16146, Genoa, Italy 2. Sezione Chimica Farmaceutica, Dipartimento di Farmacia, Università di Genova, Genoa, Italy
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Abstract: | GABAA receptor mediated inhibition plays an important role in modulating the input/output dynamics of cerebellum. A characteristic of cerebellar GABAA receptors is the presence in cerebellar granule cells of subunits such as α6 and δ which give insensitivity to classical benzodiazepines. In fact, cerebellar GABAA receptors have generally been considered a poor model for testing drugs which potentially are active at the benzodiazepine site. In this overview we show how rat cerebellar granule cells in culture may be a useful model for studying new benzodiazepine site agonists. This is based on the pharmacological separation of diazepam-sensitive α1 β2/3 γ2 receptors from those which are diazepam-insensitive and contain the α6 subunit. This is achieved by utilizing furosemide/Zn2+ which block α6 containing and incomplete receptors. |
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