Production of betalains by suspension cultures of Chenopodium rubrum L. |
| |
Authors: | Jochen Berlin Sabine Sieg Dieter Strack Maria Bokern Hans Harms |
| |
Affiliation: | (1) GBF-Gesellschaft f. Biotechnologische Forschung m.b.H., D-3300 Braunschweig;(2) Botanisches Institut der Universität, D-5000 Köln;(3) Institut f. Pflanzenernährung, Bundesforschungsastalt f. Landwirtschaft, D-3300 Braunschweig, FRG |
| |
Abstract: | Red-violet cell suspension cultures of Chenopodium rubrum were found to accumulate the betacyanins amaranthin, celosianin and betanin and the betaxanthins vulgaxanthin I and vulgaxanthin II. Under a 16-h daylight regime the cells accumulated 0.3–0.4% betacyanins on a dry mass basis after 2–3 weeks of cultivation on the growth medium. Experiments to define a production medium for betacyanins failed with this habituated line. The accumulation could however be increased up to 1% or 100 mg betacyanins/1 by feeding tyrosine and by adaptation of the inoculum size to the nutrient concentration. |
| |
Keywords: | Chenopodium rubrum cell suspension cultures betalains accumulation media variation |
本文献已被 SpringerLink 等数据库收录! |