Cytometry and flow cytometry of 4',6-diamidino-2-phenylindole (DAPI)-stained suspensions of nuclei released from fresh and fixed tissues of plants |
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| Authors: | Sergio Sgorbati Marisa Levi Elio Sparvoli Federica Trezzi Giovanni Lucchini |
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| Affiliation: | Universitàdegli Studi di Milano, Dipartimento di Biologia, Sezione di Botanica Generale e Centro di Studio del CNR per la Biologia Cellulare e Molecolare delle Piante, Via Celoria 26, I-20133 Milano, Italy. |
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| Abstract: | Cytometry and flow cytometry were used to study characteristics of fluorescence of the DNA-DAPI complex in nuclei released from different fresh and formaldehyde-fixed pea ( Pisum sativum L. cv. Lincoln) tissues. The two methods of isolation are compared and discussed as well as their possible use for quantitative analysis of DNA in plant tissues. With fixed tissues it is possible to obtain a number of nuclei sufficient for the flow cytometric analysis, even using small amounts of plant tissue. |
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| Keywords: | Cell cycle chicken erythrocytes DNA content fluorochromes formaldehyde fixation pea |
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