Cloning of genes for a hemolytic factor of Leptospira interrogans serovar autumnalis strain Congo 21-543 |
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Authors: | M Fukunaga I Mifuchi Y Yanagihara |
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Affiliation: | Faculty of Pharmacy and Pharmaceutical Science, University of Fukuyama. |
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Abstract: | A DNA fragment encoding a hemolytic factor was cloned from the parasitic spirochete Leptospira interrogans serovar autumnalis strain Congo 21-543. Initial clones were isolated by screening a genomic library in pBR322 in Escherichia coli for hemolytic activity. Hemolytic activity was coded by a 4.5 kilobase BamHI-HindIII fragment. Southern hybridization with DNAs from other strains of Leptospira using this gene as a probe showed that DNAs from non-parasitic strains failed to hybridize with the probe, whereas those from all parasitic strains tested had the sequence which hybridize to the probe. |
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